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Wednesday, 20 July 2011

An old classic

Hilarious!!! So catchy I almost want to sing along. Haha,, who's your daddy!?!!??!

Lyrics
The PCR Song by Scientists for Better PCR
There was a time when to amplify DNA,
You had to grow tons and tons of tiny cells.
(Oooh) Then along came a guy named Dr. Kary Mullis,
Said you can amplify in vitro just as well.

Just mix your template with a buffer and some primers,
Nucleotides and polymerases too.
Denaturing, annealing, and extending,
Well it’s amazing what heating and cooling and heating will do.

[Chorus]
PCR when you need to detect mutation (detect mutation)
PCR when you need to recombine (recombine)
PCR when you need to find out who the daddy is (who’s your daddy?)
PCR when you need to solve a crime (solve a crime)
[x2]

Terms simplified
polymerase chain reaction (PCR) - scientific technique in molecular biology to amplify a piece of DNA across several orders of magnitude. 

in vitro - in a plate

template - small piece of DNA that you want to amplify e.g. DNA of Bill Clinton from semen on Lewinsky's dress!

primers - short sequence of DNA that matches the beginning/end of the region on the template that you want to amplify

nucleotides - DNA is made up of a chain of nucleotides

polymerases - enzyme that links nucleotides together to make DNA

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